Grease the sides of each three 8-inch or 9-inch round cake pan with butter or nonstick spray.
Line the bottoms with parchment paper rounds to prevent sticking and ensure clean removal after baking.
Heat your oven to 350°F (175°C) so it’s ready when the batter is done.
In the bowl of a stand mixer fitted with the paddle attachment, beat the softened butter and granulated sugar together on medium speed until light, fluffy, and pale in color, about 3–5 minutes.
Stop halfway through to scrape down the sides and bottom of the bowl so everything mixes evenly.
In a medium mixing bowl, whisk together the eggs, egg whites, sour cream, milk, and vanilla extract until smooth and well combined.
In another medium bowl, whisk together the gluten free flour blend, potato starch, guar gum (if using), baking powder, baking soda, and salt.
This helps prevent clumps and ensures the leavening agents are evenly distributed.
Reduce the stand mixer speed to medium-low.
Add the dry and wet ingredients alternately to the butter-sugar mixture: begin with 1/3 of the wet, then 1/3 of the dry, repeating until all is incorporated, and ending with the wet mixture.
Mix only until just combined; do not overmix or the cake may become dense. The batter will be thick but smooth.
Gently toss the blueberries with 1 tablespoon of the dry flour mixture to coat them lightly (this prevents them from sinking).
Fold the blueberries into the batter with a spatula until evenly distributed.
Divide the batter evenly among the prepared cake pans. Use an offset spatula or the back of a spoon to spread the batter into an even layer and smooth the tops.
Bake for 15 minutes, rotate the pans for even baking, then continue baking for another 15–20 minutes.
The cakes are done when the tops are golden brown and a toothpick inserted into the center comes out with only a few moist crumbs (no wet batter).
Remove pans from the oven and allow cakes to cool in the pans for about 15 minutes.
Run a thin knife around the edges if needed, then carefully invert the cakes onto wire racks.
Peel off parchment paper and allow the cakes to cool completely before frosting.